In this study, a new empirical approach was developed to evaluate changes in asphalt concrete microstructure under full-scale accelerated pavement test loading with a Heavy Vehicle Simulator (HVS) of composite pavement. The approach compared X-ray computed tomography (CT) images taken before and after HVS rut testing. Results were used to identify the differences in the movement of aggregate and changes in air-void content and distribution occurring during rutting accumulation of rubberized gap graded and polymer modified dense graded mixes for two overlay thicknesses (64 and 114 mm).